Composite
Part:BBa_K209468:Design
Designed by: Katja Kolar Group: iGEM09_UCSF (2009-10-05)
pCEP-SSFYFP-hM4D-ActA(30-612)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6034
Illegal EcoRI site found at 8330
Illegal EcoRI site found at 8726
Illegal XbaI site found at 609
Illegal XbaI site found at 1146
Illegal SpeI site found at 18
Illegal SpeI site found at 2057
Illegal PstI site found at 6786
Illegal PstI site found at 8423
Illegal PstI site found at 8816
Illegal PstI site found at 10083
Illegal PstI site found at 10612
Illegal PstI site found at 11316
Illegal PstI site found at 12111 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6034
Illegal EcoRI site found at 8330
Illegal EcoRI site found at 8726
Illegal NheI site found at 630
Illegal NheI site found at 642
Illegal NheI site found at 12347
Illegal NheI site found at 14003
Illegal SpeI site found at 18
Illegal SpeI site found at 2057
Illegal PstI site found at 6786
Illegal PstI site found at 8423
Illegal PstI site found at 8816
Illegal PstI site found at 10083
Illegal PstI site found at 10612
Illegal PstI site found at 11316
Illegal PstI site found at 12111
Illegal NotI site found at 646 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6034
Illegal EcoRI site found at 8330
Illegal EcoRI site found at 8726
Illegal BglII site found at 604
Illegal BglII site found at 10111
Illegal BglII site found at 10394
Illegal BamHI site found at 745
Illegal BamHI site found at 12840
Illegal XhoI site found at 727 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6034
Illegal EcoRI site found at 8330
Illegal EcoRI site found at 8726
Illegal XbaI site found at 609
Illegal XbaI site found at 1146
Illegal SpeI site found at 18
Illegal SpeI site found at 2057
Illegal PstI site found at 6786
Illegal PstI site found at 8423
Illegal PstI site found at 8816
Illegal PstI site found at 10083
Illegal PstI site found at 10612
Illegal PstI site found at 11316
Illegal PstI site found at 12111 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6034
Illegal EcoRI site found at 8330
Illegal EcoRI site found at 8726
Illegal XbaI site found at 609
Illegal XbaI site found at 1146
Illegal SpeI site found at 18
Illegal SpeI site found at 2057
Illegal PstI site found at 6786
Illegal PstI site found at 8423
Illegal PstI site found at 8816
Illegal PstI site found at 10083
Illegal PstI site found at 10612
Illegal PstI site found at 11316
Illegal PstI site found at 12111
Illegal NgoMIV site found at 733
Illegal NgoMIV site found at 12218
Illegal NgoMIV site found at 13871
Illegal AgeI site found at 681 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5402
Illegal BsaI site found at 6960
Illegal BsaI site found at 10350
Illegal BsaI.rc site found at 4440
Illegal BsaI.rc site found at 9660
Illegal SapI.rc site found at 12621
Design Notes
Cloned into BBa_K209432.